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Oxidation of Zn(II)/S clusters or reaction with reactive oxygen/nitrogen species leads to partial Zn(II) dissociation due to structural changes and lack of Zn(II) affinity to disulfides (Krel and Maret 2007a)

The procedure involved extracting blood samples from filter paper using methanol, followed by drying the supernatant under nitrogen at 40 C, derivatization with butanolic-HCl, and reconstitution with 200 L of acetonitrile-water (50:50 v/v) containing 0.05 % formic acid to adjust pH to 4 for LC-MS/MS analysis

Ninety and two percent of them were HBeAg negative

Ethylenediaminetetraacetic acid (EDTA) is approved by the U.S

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