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Based on the nucleotide sequences of the target genes, development of primer sets were designed and used to amplify gene fragments by PCR (Additional file 1: Tables S1S6)

~ AlphaMD As men age, particularly after 50, natural testosterone levels can decline, leading to various physical and psychological changes

AOD9604 is a synthetic peptide fragment derived from human growth hormone, specifically encompassing amino acids 176191 of the hormones sequence

Given the shared microbiota changes in GD and depression (26, 150154), as well as the potential effects of microbiota dysbiosis in GD on depression, probiotics and symbiotics may exert beneficial effects in improving the intestinal flora environment of patients with GD, thus reducing the risk of depression