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Future studies examining protein expression and activation, such as Western blot analysis of phosphorylated Akt and PI3K, are warranted to confirm the mechanistic effects of DHA and Vildagliptin on the SIRT1/Akt/PI3K pathway in insulin-sensitive tissues such as skeletal muscle, liver, and adipose tissue to strengthen mechanistic and translational relevance

& Yanker, B.A

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Moreover, a strong effect of the fasting intervention was observed in relation to amylase concentration decrease, indicating a significant reduction in carbohydrate stores in the men tested under these conditions