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To purify only RBD we made use of the TEV cleavage site in between the RBD and the GST tag

subsequently, ASC recruits caspase-1 via filamentous CARD-CARD interactions, which solidifies the condensation and reduces their fluidity, ultimately resulting in the formation of mature inflammasomes (Fig

10.1038/s41598-020-72635-x 150 MaY.ChenH.JiangY.WangD.AschnerM.LuoW.et al (2024)

substituted or unsubstituted, cyclic or acyclic, branched or unbranched C 1-20 An aliphatic group

4.2 Immune cell crosstalk: amplifying the axis cycle Immune cells play a dual role in asthma, both responding to and actively promoting ferroptosis, thereby fueling a cycle of inflammation and cell death