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HUVECs and NIH 3T3 cells in Roswell Park Memorial Institute (RPMI) 1640 and HaCaT in Dulbeccos Minimum Essential Medium (DMEM)/F-12 medium were cultured in the indicated media supplemented with 10% fetal bovine serum (FBS) and maintained at 37C in a humidified environment with 5% CO 2

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7b, c), their differentiation into CC1 + /Olig2 + OLs was impaired by hypoxia but this difference with normoxic group was rescued by both dyclonine and leucovorin treatments, with leucovorin also reaching statistical difference with the hypoxic group (Fig

To view a copy of this licence, visit About this article Cite this article Samavat, J., Boachie, J., McTernan, P.G