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Some injuries require surgery

In salmonids, acute stress has been shown to transport intestinal mucin towards the anus (Olsen et al., 2005, Olsen et al., 2008), exhaust eicosanoid cascades (Olsen et al., 2012) and rupture of junctional complexes eventually leading to impaired barrier functions that may last for several days after exposure (Olsen et al., 2002, Olsen et al., 2005)

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Theres no strict limit, but there are concerns about repeated injections in the same area