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If IM injections arent right for you, well discuss other options

The phospho-Met antibody were added to the blocking buffer at a final concentration of 1 : 1000 and incubated at 4C overnight with gentle agitation

PLoS ONE 9:e86656

Standard practice includes: Allow the sealed vial to reach room temperature approximately 15-30 minutes Clean the rubber stopper with an alcohol swab Add the calculated volume of solvent to achieve desired concentration Gently swirl or roll the vial between palms do not shake vigorously to avoid foaming Allow complete dissolution before use this typically takes 2-5 minutes Reconstitution Example for AOD9604 10MG: For researchers needing a 10mg/mL working solution, add 1mL of sterile solvent to the 10mg vial