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Lysates were separated by 10% SDS-PAGE and 415% precast gradient gels (Biorad)

peptide is hygroscopic Reconstitute in sterile water or appropriate buffered aqueous solution depending on assay requirements Avoid vigorous agitation during dissolution Filter sterilize if required (e.g

BPC-157 remains soluble and active in aqueous research media from pH 1 to pH 11, an exceptional range that reflects both the proline-driven rigidity and the absence of titratable side chains that would normally drive aggregation at extreme pH