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Enzymatic Reaction Conditions The substrate solution used for the NsPCS reaction was prepared as follows: 28.7 mg of tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Tokyo Chemical Industry) and 3.0733 g of GSH (Nacalai Tesque) were weighed in a 100 mL volumetric flask, 20 mL of 1000 mM potassium phosphate buffer (pH 8.0) was added, and the solution was gently shaken

Of relevance is that the area under the curve of the full plasma levels of ribavirin for the different formulations (0-2% GSH) was not significantly different

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Clinical studies show oral NR increases NAD+ concentrations more than twofold compared to placebo (47.75 M versus 20.90 M) without raising blood NAM levels