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Acknowledgements The authors thank the women who took part in this study and all the sta at the Reproductive Medicine Center, Renmin Hospital, Hubei University of Medicine

For purification, harvested cells were first re-suspended in chilled lysis buffer (20 mM Tris [pH 7.9], 0.5 M NaCl, 5 mM imidazole, 10% glycerol, 1 mM DTT, 1 mM PMSF) and the lysis mixture was sonicated on ice