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After 12 h of culture, the medium was discarded, and 10 L of MTT solution (5 mg/mL) was added to each well and incubated for 4 ~ 6 h

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This strategy was used to quantify Flx and Ser with a detection limit of 0.5110.543 nM in an aqueous medium and 0.0410.047 nM in a polyvinylpyrrolidone (PVP) micellar medium without any sophisticated equipment

and Angelica keiskei (Miq.) Koidz