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Shamsad A, Gautam T, Singh R, Banerjee M

Proteins of interest were probed with primary antibodies against GS (Millipore, MAB302), YAP (Cell Signaling Technology, D8H1X), phospho-YAP (Ser127, Cell Signaling Technology, D9W2I), -tubulin (Sigma-Aldrich, T5168), GAPDH (Santa Cruz Biotechnology, SC365062), Lamin B1 (Santa Cruz Biotechnology, SC377000), LATS1 (Cell Signaling Technology, C66B5), phospho-LATS1 (Ser909, Cell Signaling Technology, 9157), MST1 (Cell Signaling Technology, 3682), phospho-MST1/2 (Cell Signaling Technology, E7U1D), then probed with horseradish peroxidaseconjugated secondary antibodies (GE Healthcare) and visualized by ECL (SuperSignal West Dura Extended Duration Substrate, Thermo Fisher Scientific)

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A human serum albumin solution (Cormay, Poland) was used as a standard

Our in vivo findings provide evidence that the glutathione metabolism pathway contributes to ESCC progression via inhibition of the NF-kB signaling pathway and provides a groundwork for further development of a glutathione metabolism inhibitor to treat ESCC