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An aliquot (2.5 l) of the amplicon obtained from the first reaction was added to the reaction mixture to give a final volume of 25 l

Parkin modulates activity of redox state-sensitive, mitochondrial enzymes To link our redox chemistry findings back to the identified changes from proteomic screens conducted in parkin-deficient mouse brain [36, 38], we explored the activities of select, redox-sensitive enzymes