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Immunohistochemistry Under deep inhalation of sevoflurane (Maruishi Pharmaceutical Company), rodents were transcardially perfused with 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.2)

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revealed that CKD patients had poorer clinical cardiovascular outcomes in association with enhanced inflammation and oxidative stress, as evidenced by their elevated MDA-modified LDL levels and peak serum C-reactive protein (CRP) [43]

Cell death at this time point was indiscriminate, as the lesion site was largely devoid of neuronal (NeuN + ), astrocytic (GFAP + ), oligodendrocyte (APC + ), and microglia (Iba1 + ) staining (all in green)