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Third, adapting these methods for use with microplate readers is complicated due to the need for protein precipitation

The activated signaling pathways promote the release of pro-inflammatory cytokines, leading to further aggravation of RA (227, 228)

Tateishi, K

Consistently, GSH depletion or silencing of Fe-S cluster-associated GLRXs disrupts mitochondrial redox balance, promotes iron overload, and sensitizes cancer cells to ferroptosis 133,134

Cells were seeded in 24-well plates and co-transfected with PARN-WT or PARN-MUT reporter plasmids together with a Renilla luciferase plasmid as an internal control, using Lipofectamine 3000 (Thermo Fisher Scientific)