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Only dark, brown-stained cells were counted

For Oil Red O staining, slides were rinsed with 60% isopropanol (Carl Roth 6752) for 10 min followed by incubation with Oil Red O (Sigma Aldrich O0625) staining solution (0.5 g Oil Red O in 100 ml isopropanol) for 15 min

The National Cancer Institute projected that in 2025, more than 2 million new cases of cancer will be diagnosed and 618,120 people will die from the disease in the United States