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To measure the biochemical parameters, the tissues were perfused with a phosphate-buffered saline (PBS) solution (pH 7.4) containing 0.16 mg/ml heparin at the time of measurement

The L-carnitine dose was selected based on prior research (Samir et al., 2018) that demonstrated its optimal efficacy in reducing oxidative stress in rodent models, while maintaining a favorable safety profile

The activity of melatonin receptors may be associated with the activation of antioxidant genes, such as superoxide dismutase and catalase, through a process initiated by receptor-mediated transcriptional regulation

Patient sample Patient tissues were obtained from HCC patients who underwent surgery at Chungnam National University Hospital

del Rio, L.A