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In the present study, a versatile green approach was designed for the preparation of HAp using seashells as green calcium precursors, and Ixora chinensis flower extract as a green reagent under low processing temperature

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For serum analysis of insulin, adiponectin, and leptin levels, serum was obtained by centrifugation of blood (10,000 g , 10 min, 4) and assayed using an ELISA Kit (Millipore, Billerica, MA)

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