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However, also the density at 60 F (15.6C) or 20 C is often used as the reference temperature, e.g

Metformin attenuates blood-brain barrier disruption in mice following middle cerebral artery occlusion

Front Immunol 15:1375589 Czuba LC, Hillgren KM, Swaan PW (2018) Post-translational modifications of transporters

PLC Another class of phospholipases catalyzing the hydrolysis of the ester bond between glycerol and the phosphate residue are the phospholipases PLC, which are activated by calcium ions, heterotrimeric large G proteins, small G proteins, and receptor/nonreceptor tyrosine kinases [72]

Bound proteins were washed several times, then eluted from beads with Flag peptide at 200 g/ml in wash buffer (Sigma) and incubated with Strep-Tactin beads in wash buffer at 4 C overnight