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10.1006/bbrc.2001.6052 127

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The blocking solution was removed and 150 L primary antibody (711,500, Rabbit Occludin Polyclonal Antibody, ThermoFisher Scientific) diluted in blocking solution (8.33 g/mL) was added

Despite numerous studies exploring the effects of Met supplementation in dairy and beef cattle, findings often vary, likely attributed to differences in experimental methodologies, RPM sources, and the developmental stages of the animals (Wei et al., 2022)