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Belujon and Grace (2017) systematically elaborated in their review on the central role of dopamine system dysregulation in MDD, noting that anhedonia, a core symptom of depression, is closely related to mesolimbic dopamine pathway dysfunction

H226, H460 and H1975 cells were grown in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% FBS, 100 IU ml 1 penicillin and 100 g ml 1 streptomycin

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To test that hypothesis we used phallodin, the toxin found in cap mushroom, known to bind specifically at the interface of F-actin subunits (Figure 5)

Statistical analyses were conducted using GraphPad Prism, with comparisons made using Students t -test (for two groups), one-way ANOVA (for three or more groups with one variable), or two-way ANOVA (with multi variables), as appropriate