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Is this only for severe conditions

Toxicol Appl Pharmacol 204(3):343

The homogenate was filtered through a 100-M cell strainer and centrifuged at 1,000 g for 10 min at 4 C, then the nuclear pellet was washed once in nucleus preparation buffer followed by resuspension in PBS with 1% BSA and 0.2 U l Roche Protector RNase inhibitor

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To aid and support the diagnosis, especially in cases of uncertainty, a definitive diagnosis is often confirmed with skin biopsy