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For transfection, each well was treated with a complex formed by combining 2.0 g of the pHaloTag-PS plasmid (purchased from Beijing Solarbio Science & Technology Co., Ltd.) with 6 L of Lipofectamine 3000 transfection reagent in 100 L of Opti-MEM I reduced serum medium, according to the manufacturers protocol

Our observations are consistent with a previous clinical study in solid tumors where continuous infusion of BSO depleted tumor GSH below 10% of pretreatment levels with minimal systemic toxic effects

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If youve been sick with the flu, food poisoning, or a stomach virus, a few sessions spaced over one to two weeks can replenish lost fluids, restore electrolyte balance, and accelerate recovery

To identify potential outliers, principal component analysis was performed after log transformation and Pareto scaling were applied to the entire dataset using MetaboAnalyst 5.0 online software ( Two control samples were identified as outliers and removed from the dataset before further statistical analyses were performed