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While NMDA receptors allow entry of Ca 2+ , AMPA receptors heterotetramer, which consists of four glutamate receptor subunits (GluR1-4), mediate the entry of Na + current into the neuron, thus resulting in rapid signal transmission ( Ketamine: One Drug, Many Uses Originally approved in 1970 as an anesthetic, ketamine thereafter earned notoriety as a recreational drug of abuse (Special K) because of its dissociative effects

Cell lysis was performed by immersing the slides in lysis buffer (2.5 M NaCl, 100 mM Na 2 EDTA, 10 mM Tris Base, 1% Triton X-100, 10% DMSO, pH 10) at 4 C for 1 h or overnight

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J Pharmacol Exp Ther 279, 14701483 (1996)

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