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However, if iron was incubated simultaneously with apoE, the iron would not have time to be shunted to ferritin, but rather would be available to induce lipid peroxidation without the need for ferritinophagy, and therefore simultaneous iron co-treatment would abolish apoE protection against ferroptosis

10.1073/pnas.222546799 57 FremeauR

This ratio is in the range of 500

Upon elution of the supermix bound fraction, both columns were neutralized and re-equilibrated by flowing 100% Buffer C (1X Seppro Neutralization Buffer, Sigma) in tandem over both columns at 2 mL/min for 18 min, followed by re-equilibration with 100% Buffer A for 20 min