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Other factors to consider Not drinking is the safest option if you are: involved in, or supervising, risky activities including driving, operating machinery or water sports supervising young people You should get advice from your doctor about drinking if: you are taking any medicines, including prescription or over-the-counter medicines you have an alcohol-related or other physical condition, that can be affected by alcohol you have mental health issues You may have an increased risk of harm if you: are under 18 years of age are over 60 years of age have a family history of alcohol dependence use illegal drugs

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Note C-terminal 6xHIS tag (underlined) is included in the pET21 bacterial expression vector

We also learned that cytotoxicity can be enhanced by other agents, such as lipoic acid, and magnetic fields