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Hopkins biographer, Dr Alison Thomas from Anglia Ruskin University, commented: At a time when there was virtually no women researchers in any Cambridge department, Hopkins gave them opportunities, despite the hostile criticism this often brought him. Hopkins also made an important contribution in the rescuing of scientists from Nazi Germany during the inter war period

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The investigators were not blinded to mouse allocation during the experiments or outcome assessments

It is also the primary source of ROS, whose heighten accumulation can lead to biomolecular impairment and cell death

Additionally, the protocol does not require a dialysis step when switching from the GST to the His-purification step column (TALON resin)