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As whole blood possesses high levels of ET, we investigated whether the ET detected in the tissues was present within the tissue itself or resulted from blood remaining in the tissue after washing

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Briefly, the yeast cell pellet was collected by centrifugation in a microcentrifuge tube and washed three times with sterile water, then quenched with pre-chilled 80% methanol (kept at 80C), followed by seven cycles of bead beating (using glass beads)

Electrohydrodynamic encapsulation of probiotics: a review