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The fractions containing eluted protein were loaded onto a 5 ml HiTrap Heparin HP affinity column (Cytiva) pre-equilibrated in H buffer containing 150 mM NaCl and eluted with an increasing salt gradient to 1 M NaCl

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As such, they have been quantified in models of inflammatory disease, like atherosclerosis, to identify relationships between disease progression and oxidative damage ( Reference Lakshmi, Padmaja and Kuppusamy 54 )

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