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The PCR protocol included a step of denaturation at 95C for 30 s, followed by 40 cycles of 95C for 5 s and 60C for 31 s

Durand CM, Betancur C, Boeckers TM, Bockmann J, Chaste P, Fauchereau F, et al

Details of the reaction conditions, CpG sites assayed, and target site primers (Supplementary Table S3) are included in the supplementary methods

[Abstract]2025 Sep 16:e202511471

The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates